Team:Warsaw/Calendar-Main/19 May 2008

From 2008.igem.org

(Difference between revisions)
Line 1: Line 1:
{{WarNotebook}}
{{WarNotebook}}
<!-- do not edit above me! -->
<!-- do not edit above me! -->
-
<html><h3>PCRs for fusions</h3>
+
<html><h3>PCRs for fusions<br>
-
<h4>Michał K.:</h4>
+
Michał K.</h3>
<p>
<p>
<ol>
<ol>
Line 25: Line 25:
<li>Electrophoresis to estimate the concentration of isolated DNA.</li></ol></p>
<li>Electrophoresis to estimate the concentration of isolated DNA.</li></ol></p>
<img src="https://static.igem.org/mediawiki/2008/e/e5/Pcr_fusion_WAW.jpg" width=300/>
<img src="https://static.igem.org/mediawiki/2008/e/e5/Pcr_fusion_WAW.jpg" width=300/>
-
<h3>1-DNA ladder; 2-PCR product-translation fusion</h3>
+
<var>1-DNA ladder; 2-PCR product-translation fusion</var>
-
</html>
+
 
 +
 
-
<html>
 
<h4>Michał L., Ewa, Marcin:</h4>
<h4>Michał L., Ewa, Marcin:</h4>
-
<ol><li>Overnight inoculation and induction:</li>  
+
<p>Overnight inoculation and induction:<br>
-
<p><a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">Top10</a> + 0,5 mM IPTG</p>
+
<a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">Top10</a> + 0,5 mM IPTG<br>
-
<p>pMPM-AID + Glc + 0,01% L-Ara</p>
+
pMPM-AID + Glc + 0,01% L-Ara<br>
-
<p><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pTrc99A-AID>pTrc99a-AID</a> + Glc + 0,5 mM IPTG</p>
+
<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pTrc99A-AID>pTrc99a-AID</a> + Glc + 0,5 mM IPTG<br>
</html>
</html>

Revision as of 14:12, 9 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


PCRs for fusions
Michał K.

  1. PCR - translation fusion: AID + T7 RNA-polymerase
    Primers:
    Template DNA: purified PCR products from 16 May - AID and T7 RNA-polymerase for translation fusion
    Annealing temperature : 73°C
    Elongation time: 4 minutes
    20 cycles
  2. Gel electrophoresis and isolation of proper band (3300 bp).
  3. Electrophoresis to estimate the concentration of isolated DNA.

1-DNA ladder; 2-PCR product-translation fusion

Michał L., Ewa, Marcin:

Overnight inoculation and induction:
Top10 + 0,5 mM IPTG
pMPM-AID + Glc + 0,01% L-Ara
pTrc99a-AID + Glc + 0,5 mM IPTG

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/19_May_2008"