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Team:ESBS-Strasbourg/6 October 2008
From 2008.igem.org
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** Ura3 (FI with Pst1 site) + pSB1A2 (FV) | ** Ura3 (FI with Pst1 site) + pSB1A2 (FV) | ||
** Leu2 (BI with EcoRI site) + pSB1A2 (BV) | ** Leu2 (BI with EcoRI site) + pSB1A2 (BV) | ||
| + | ** Ura3 (FI with Pst1 site) + LexAo8_cyc100_Kozak_CFP_NLS_adhterm (''alias Reporter cyc100'') (FV) | ||
| + | ** Ura3 (FI with Pst1 site) + LexAo8_cyc16_Kozak_CFP_NLS_adhterm (''alias Reporter cyc16'') (FV) | ||
| + | ** Ura3 (FI with Pst1 site) + LexAo8_cyc43_Kozak_CFP_NLS_adhterm (''alias Reporter cyc43'') (FV) | ||
** Gal1 (BV)+ CFP_NLS_adhterm (BI) | ** Gal1 (BV)+ CFP_NLS_adhterm (BI) | ||
** Gal1 (BV)+ CFP_cln2_adhterm (BI) | ** Gal1 (BV)+ CFP_cln2_adhterm (BI) | ||
| Line 18: | Line 21: | ||
* Preparation of LB-media (2x150ml, 2x100ml) | * Preparation of LB-media (2x150ml, 2x100ml) | ||
* Start of ON of lexA_L, mCherry_L_VP16_L, cin8_adhterm | * Start of ON of lexA_L, mCherry_L_VP16_L, cin8_adhterm | ||
| - | + | <br\n> | |
''goals for tomorrow:'' | ''goals for tomorrow:'' | ||
* Verification of: | * Verification of: | ||
| - | ** Ura3 | + | ** Ura3 with |
** Leu2 | ** Leu2 | ||
** Gal1_CFP_NLS_adhterm | ** Gal1_CFP_NLS_adhterm | ||
Revision as of 17:52, 6 October 2008
Contents |
DryLab
Modeling
WetLab
Katja:
- Verification PCR for Leu2 and Ura3 parts -> negative
- Ligation and Transformation of:
- Ura3 (FI with Pst1 site) + pSB1A2 (FV)
- Leu2 (BI with EcoRI site) + pSB1A2 (BV)
- Ura3 (FI with Pst1 site) + LexAo8_cyc100_Kozak_CFP_NLS_adhterm (alias Reporter cyc100) (FV)
- Ura3 (FI with Pst1 site) + LexAo8_cyc16_Kozak_CFP_NLS_adhterm (alias Reporter cyc16) (FV)
- Ura3 (FI with Pst1 site) + LexAo8_cyc43_Kozak_CFP_NLS_adhterm (alias Reporter cyc43) (FV)
- Gal1 (BV)+ CFP_NLS_adhterm (BI)
- Gal1 (BV)+ CFP_cln2_adhterm (BI)
- Gal1 (BV)+ CFP_hsl1_adhterm (BI)
- Gal1 (BV)+ mCherry_L_lexA_L_VP16_L (BI) (Ligation by Sandra)
- Gal1 (BV)+ lexA_L_VP16_L_mCherry_L (BI) (Ligation by Sandra)
- Preparation of LB-media (2x150ml, 2x100ml)
- Start of ON of lexA_L, mCherry_L_VP16_L, cin8_adhterm
goals for tomorrow:
- Verification of:
- Ura3 with
- Leu2
- Gal1_CFP_NLS_adhterm
- Gal1_CFP_cln2_adhterm
- Gal1_CFP_hsl1_adhterm
- Gal1_mCherry_L_lexA_L_VP16_L
- Gal1_lexA_L_VP16_L_mCherry_L
General
There are only 10 aliquotes of competent TOP10 cells left!
