Team:Warsaw/Calendar-Main/18 June 2008

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<h4>Michał L., Ewa, Marcin:</h4>
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<h3>Preparation of constructs with OmpA protein fusions<br>
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Michał K.</h3>
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<li>Counting of blue colonies. Same results as on <a href=https://2008.igem.org/Team:Warsaw/Calendar-Main/12_June_2008>12/06/2008</a>. </li></ol><h3>Preparation of constructs with OmpA protein fusions</h3>
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<h4>Michał K.:</h4>
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<ol><li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of pACYC177.</li>
<ol><li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of pACYC177.</li>
<li><a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">Digest</a> of purified pACYC177 with NdeI and BamHI. </li>  
<li><a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">Digest</a> of purified pACYC177 with NdeI and BamHI. </li>  
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<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of purified DNA (pACYC177 with OmpA_alpha and OmpA_omega DNA fragments).</li>
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of purified DNA (pACYC177 with OmpA_alpha and OmpA_omega DNA fragments).</li>
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <i>E.coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> with ligation products.</li>
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <i>E.coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> with ligation products.</li>
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<li>Plating transformants on LB+tetracycline.</li></ol></html><br>
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<li>Plating transformants on LB+tetracycline.</li></ol>  
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<h4>Michał L., Ewa, Marcin:</h4>
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<p>Counting of blue colonies. Same results as on <a href=https://2008.igem.org/Team:Warsaw/Calendar-Main/12_June_2008>12/06/2008</a>. </p>
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Revision as of 15:20, 9 October 2008

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Preparation of constructs with OmpA protein fusions
Michał K.

  1. Isolation of pACYC177.
  2. Digest of purified pACYC177 with NdeI and BamHI.
  3. Gel electrophoresis and isolation of proper band (3200 bp).
  4. Ligation of purified DNA (pACYC177 with OmpA_alpha and OmpA_omega DNA fragments).
  5. Transformation of E.coli TOP10 with ligation products.
  6. Plating transformants on LB+tetracycline.

Michał L., Ewa, Marcin:

Counting of blue colonies. Same results as on 12/06/2008.

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