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Team:Illinois/Antibody GPCR Fusion Notebook
From 2008.igem.org
(Difference between revisions)
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!-- == 25th August == | !-- == 25th August == | ||
| - | * Prepared overnight culture for DNA extraction (3:27pm)-- | + | * Prepared overnight culture for DNA extraction (3:27pm) |
| + | |||
| + | |||
| + | !-- == 26th August == | ||
| + | * Attempted DNA extraction | ||
| + | * Prepped overnight culture | ||
| + | |||
| + | |||
| + | !-- == 27th August == | ||
| + | * Performed PCR (FILL IN PCR TABLE) | ||
| + | * Prepped 3 overnight cultures | ||
| + | |||
| + | |||
| + | !-- == 28th August == | ||
| + | * Extracted DNA from 4 cultures | ||
| + | * Ran gel of PCR products (1.5%, 200V) | ||
| + | ** Result: No bands present | ||
| + | |||
| + | |||
Revision as of 20:15, 7 October 2008
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!-- == 22nd July ==
- Yeast obtained from Dr. Zhao
!-- == 24th July ==
- Prepared liquid culture for DNA extraction
- Made 1M Tris. Cl pH 8.0
- Made 4M ammonium acetate
!-- == 22nd August ==
- Attempted DNA extraction
- Result: Failed
- Obtained more yeast from Dr. Zhao
!-- == 25th August ==
- Prepared overnight culture for DNA extraction (3:27pm)
!-- == 26th August ==
- Attempted DNA extraction
- Prepped overnight culture
!-- == 27th August ==
- Performed PCR (FILL IN PCR TABLE)
- Prepped 3 overnight cultures
!-- == 28th August ==
- Extracted DNA from 4 cultures
- Ran gel of PCR products (1.5%, 200V)
- Result: No bands present
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