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Team:Warsaw/Calendar-Main/28 May 2008
From 2008.igem.org
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<li> Choice of proper clones and preparation for sequencing.</li> | <li> Choice of proper clones and preparation for sequencing.</li> | ||
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| + | <h3>Here we found the clone with translational fusion on pMPM. These are different isolations in each lane. We have been working with the AT6 ever since. <br> | ||
| + | Digest was conducted with BamHI</h3> | ||
| + | <center>https://static.igem.org/mediawiki/2008/6/62/Trawienie_06_05_2008.jpg</center> | ||
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| + | <img src="https://static.igem.org/mediawiki/2008/d/d6/Translation_fusion_digest_Eco.jpg"width=300/></div> | ||
| + | <h3>2 to 6-Control digests of few clones with transcription fusion; 7-pMPMT5-AID; all plasmids digested with EcoRI. </h3> | ||
| - | <h4> | + | <h4>Michał L., Ewa, Marcin</h4> |
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<li> Inoculation of liquid LB with E.coli <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> carrying pMPM-T5+AID </li> | <li> Inoculation of liquid LB with E.coli <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> carrying pMPM-T5+AID </li> | ||
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Revision as of 15:55, 8 October 2008
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Preparation of pMPMT5-AID+T7 and pMPMT5+AID-T7Michał K.:
Here we found the clone with translational fusion on pMPM. These are different isolations in each lane. We have been working with the AT6 ever since.
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