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Team:University of Lethbridge/Notebook/Project1October
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Revision as of 03:35, 8 October 2008
Back to The University of Lethbridge Main Notebook
Contents |
October 2, 2008
Munima, Christa, John
Objective: To set up chemotaxis spatial localization assay
Made and autoclaved media to make LB + amp plates to be used in the assay.
Recipe:
- Peptone 5g - Yeast Extract 2.5 g - NaCl 5 g - Agar (0.25 %) 1.25 g - dH2O 500 mL
October 4, 2008
Christa
Objective: To set up chemotaxis spatail locatization assay
Media made on October 2 looked funny.
Poured 23 plates (LB + amp 100ug/mL) anyway. Stored in iGEM 4 C fridge.
October 6, 2008
Christa, Alix
Objective: Make more solutions for future chemical competency experiments.
Made 500 mL of 100 mM CaCl2 solution and 500 mL of 80mM MgCl2-20mM CaCl2 solution. They are stored on the counter under the iGEM glass cabinet in 1 L media bottles.
Selina
Objective: Create motility media stab tubes controls for motility assay.
Inoculated two 5 mL LB media tubes, one with E. coli K12 (wild-type), the other with Staphylococcus epidermis and let incubate at 37 C for ~8 hours.
Stabbed motility media (no theophylline) with subcultures of either cell type, using inoculating loops (teeny loop part, essentially a rod).
-Positive control: E. coli K12 (wild-type) -Negative control: Staphylococcus epidermis
Incubated for 48 hours at 37 C.
Selina
Streaked the "LB+ amp chemically 'competent' cells transformed with pUC19 control plasmid" plate (Aug. 26) with wild-type E. coli to test for ampicillin presence.
Incubated overnight at 37 C.
October 7, 2008
Munima, John, Roxanne
Objective: Assess the motility media stab tubes after one day of incubation.
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| Positive control: K12 | Negative control: Staphylococcus epidermis |
Side-by-side comparison:
