Team:Warsaw/Calendar-Main/16 September 2008

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<h4>Piotr</h4>
<h4>Piotr</h4>
<p>Testing of <ul><li>resistance to antibiotics achieved by interacting with a protein in medium;</li><li>minimum amount of protein in medium providing resistance to ampicillin (50 ul/ml) for OmpA with A-alpha, A-omega and Z-omega;</p></li>
<p>Testing of <ul><li>resistance to antibiotics achieved by interacting with a protein in medium;</li><li>minimum amount of protein in medium providing resistance to ampicillin (50 ul/ml) for OmpA with A-alpha, A-omega and Z-omega;</p></li>
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</ul>
Tests for ampicillin resistance given by protein added to the medium:
Tests for ampicillin resistance given by protein added to the medium:

Revision as of 16:43, 8 October 2008

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Piotr

Testing of

  • resistance to antibiotics achieved by interacting with a protein in medium;
  • minimum amount of protein in medium providing resistance to ampicillin (50 ul/ml) for OmpA with A-alpha, A-omega and Z-omega;</p>

Tests for ampicillin resistance given by protein added to the medium:

Checking the minimal amount of protein added to the medium is sufficient for ampicillin (Ap 50) resistance for OmpA variants: A-alpha, A-omega and Z-omega


Rivalry Tests: Inoculation to one tube A-omega, omega and Z-omega - A_alpha protein added ~100 μg 

      (0,25IPTG; 50AP)            A-omega, omega and Z-omega - Z_alfa protein added ~100 μg 
                                  A-alpha, alpha and Z-alpha - Z_omega protein added ~100 μg


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