Piotr

Testing of

• resistance to antibiotics achieved by interacting with a protein in medium;
• minimum amount of protein in medium providing resistance to ampicillin (50 ul/ml) for OmpA with A-alpha, A-omega and Z-omega;</p>

Tests for ampicillin resistance given by protein added to the medium:

Checking the minimal amount of protein added to the medium is sufficient for ampicillin (Ap 50) resistance for OmpA variants: A-alpha, A-omega and Z-omega

Rivalry Tests: Inoculation to one tube (0,25IPTG; 50AP)

• A-omega, omega and Z-omega - A_alpha protein added ~100 μg
• A-omega, omega and Z-omega - Z_alfa protein added ~100 μg
• A-alpha, alpha and Z-alpha - Z_omega protein added ~100 μg