Team:Warsaw/Calendar-Main/29 July 2008

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(Difference between revisions)
Line 20: Line 20:
<tr><th rowspan="2">ampicillin concentration (mikrogramy na mililitr):</th><th colspan="6">IPTG concentration (milimole na mililitr):</td></tr>
<tr><th rowspan="2">ampicillin concentration (mikrogramy na mililitr):</th><th colspan="6">IPTG concentration (milimole na mililitr):</td></tr>
<tr><th>0</th><th>0,1</th><th>0,25</th><th>0,5</th><th>0,75</th><th>1</th></tr>
<tr><th>0</th><th>0,1</th><th>0,25</th><th>0,5</th><th>0,75</th><th>1</th></tr>
-
<tr><th>25</th><td class="dead">1,558</td><td class="live">1,469</td><td class="dead">1,587</td><td class="live">1,49</td><td class="dead">1,566</td><td class="live">1,311</td></tr>
+
<tr><th>25</th><td class="live">1,558</td><td class="live">1,469</td><td class="live">1,587</td><td class="live">1,49</td><td class="live">1,566</td><td class="live">1,311</td></tr>
-
<tr><th>50</th><td class="live">1,425</td><td class="dead">1,435</td><td class="live">1,524</td><td class="dead">1,055</td><td class="live">0,920</td><td class="dead">0,935</td></tr>
+
<tr><th>50</th><td class="live">1,425</td><td class="live">1,435</td><td class="live">1,524</td><td class="live">1,055</td><td class="live">0,920</td><td class="live">0,935</td></tr>
-
<tr><th>75</th><td class="dead">1.09</td><td class="live">0,989</td><td class="dead">1.447</td>
+
<tr><th>75</th><td class="live">1.09</td><td class="live">0,989</td><td class="live">1.447</td><td class="live">0.971</td><td class="live">0.951</td><td class="live">0.992</td>
</tr>
</tr>
<tfoot>
<tfoot>

Revision as of 07:55, 9 October 2008

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1. Separete transformant colonies (tranformation from previous day) inoculated to liquid LB with kanamycin.
2. Inoculation of pACYC177+OmpA_alpha and pACYC177+OmpA_omega - liquid LB with kanamycin.

Sprawdzanie czy omp_omega_A_alfa daje oporność na AP Piotr Zaszczepienie omp_omega_A_alfa z rużnych stężeń IPTG: 0; 0,1 ; 0,25 ; 0,5; 0,75; 1 do takich samych stężeń IPTG, ale z rużnymi stężeniami AP (25;50;75;100) w stosunku 1:50 wieczorem mieżenie OD :

Table 2. Results of testing various hunter/prey combinations
(ampicillin concentration 50-75 ug/ml).

ampicillin concentration (mikrogramy na mililitr):IPTG concentration (milimole na mililitr):
00,10,250,50,751
251,5581,4691,5871,491,5661,311
501,4251,4351,5241,0550,9200,935
751.090,9891.4470.9710.9510.992
Zaszczepienie omp_omega_A_alfa na rużnych stężeń IPTG: 0; 0,1 ; 0,25 ; 0,5; 0,75 (do powtużenia ) Sprawdzenie ekspresji omp_omega_A_alfa i omp_A_alfa Piotr Zwirowanie, zawieszenie, dodanie buforu lizującego (skład), zagotowanie, nałożenie na żel poliakryl, transfer na nitrocelulozę, blokowanie, przeciwciała anty-A, płukanie, przeciwciała anty-królik, wywołanie BCIP i NBT (omp_omega_A_alfa, z i bez indukcji, omp_A_alfa) (Zdjęcie żelu)

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