Team:Warsaw/Calendar-Main/29 August 2008

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<h3>Cloning of protein A DNA to pET15b+OmpA-alfa plasmid in place of OmpA<br>
 
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Antoni</h3>
 
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<p><ol><li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of pET15b+OmpA-alfa.</li></ol></p>
 
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<h3>Protein purification: A-alpha, Z-alpha i Z-omega<br>
<h3>Protein purification: A-alpha, Z-alpha i Z-omega<br>

Revision as of 15:12, 9 October 2008

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Protein purification: A-alpha, Z-alpha i Z-omega
Piotr

  1. Single transformations of Rosetta with plasmids pET15b+A-alfa, pET15b+Z-alfa and pET15b+Z-omega. Plating them on LB with Cm and Ap.

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