Team:Warsaw/Calendar-Main/25 August 2008

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<h3>Cloning of protein A DNA to pET15b+OmpA-alfa plasmid in place of OmpA<br>Antoni</h3>
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<h3>Cloning of protein A DNA to pET15b+Omp-alfa plasmid<br>Antoni</h3>
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<li> Colony PCR on colonies from plates with transformations pGeneart+A Primers used:
 
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI">AP+NotI</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL+SacI">AL+SacI</a></li>
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<li> Lack of confirmed transformant colonies </li></ol></p>
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<li> Transformation of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> strain with <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/22_August_2008">friday's</a> ligation products. </li>
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<li> Transformants plating on LB + ampicillin.</li>
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Revision as of 16:52, 12 October 2008

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Cloning of protein A DNA to pET15b+Omp-alfa plasmid
Antoni

  1. Transformation of E. coli TOP10 strain with friday's ligation products.
  2. Transformants plating on LB + ampicillin.

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