Template:Team:UC Berkeley/Notebook/DV construction

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==K112702==
==K112702==
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<pre>
 
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Construction of [<Phns>] Basic Part K112701
 
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PCR dv001/dv004 on Phns            (150 bp, gp = A)
 
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PCR dv003/dv006 on Phns            (225 bp, gp = B)
 
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PCR dv005/dv002 on Phns            (342 bp, gp = C)
 
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---------------------------------------------------
 
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PCR mv001/dv006 on A+B              (355 bp, gp = D)
 
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---------------------------------------------------
 
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PCR dv001/dv002 on C+D              (697bp, EcoRI/BamHI/DpnI)
 
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Sub into pBca1256                  (EcoRI/BamHI, 2472+697)
 
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Product is pBca1256-K112701
 
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-----------------------------------------------
 
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dv001 Forward Biobricking of [<Phns>]
 
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CCATAgaattcATGagatctGAAATATAGCTGTGCCATCAGCC
 
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dv002 Reverse Biobricking of [<Phns>]
 
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CCATAggatccGCACGAAGAGTACGGATG
 
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dv003  Removing the 1st EcoRI site in [<Phns>] (F)  GCCAGGAATGTAAGGgATTCAAAATTGTTC
 
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dv004  Removing the 1st EcoRI site in [<Phns>] (R)  GAACAATTTTGAATcCCTTACATTCCTGGC
 
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dv005  Removing the 2nd EcoRI site in [<Phns>] (F)  gctaaagaGttcgccgaagtagc
 
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dv006  Removing the 2nd EcoRI site in [<Phns>] (R)  gctacttcggcgaaCtctttagc
 
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</pre>
 
Construction of <His-Tag! Basic Part K112702
Construction of <His-Tag! Basic Part K112702

Revision as of 23:16, 10 June 2008

Contents


K112701

K112702

Construction of <His-Tag! Basic Part K112702

Mixing of dv007/dv008 (27bp, EcoRI/BamHI/DpnI) Sub into (vector?)pBca1256 (EcoRI/BamHI, 2472+27, L) Product is pBca1256-K112110

dv007 Forward biobricking of <His-Tag! CATCATCATCATCATCATtaaGGATCC dv008 Reverse biobriquing of <His-Tag! GGATCCttaATGATGATGATGATGATG


PCR dv009/dv010 on pBca9145-Bca1117 (1060bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112100

dv009 Forward Biobricking of His-Tag> ccataGAATTCatgAGATCTatgCATCATCATCATCATCAT dv010 Reverse Biobricking of His-Tag> ATGATGATGATGATGATGcatAGATCTcatGAATTCtatgg 


PCR bx1/bx2 on Bjh1301		(688 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112000
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bx1		Forward Biobricking of {T4 Holin!}
ccagtGAATTCatgAGATCTatggcagcacctagaatatc
bx2		Reverse Biobricking of {T4 Holin!}
cgttaGGATCCttatttagcccttcctaatattctggccgcc
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