Team:Warsaw/Calendar-Main/3 September 2008

From 2008.igem.org

(Difference between revisions)
Line 3: Line 3:
<html>
<html>
-
<h3>Purification of proteins:  A-alpha, Z-alpha and Z-omega<br>Piotr</h3>
+
<h3>Purification of proteins:  A-alpha, Z-alpha and Z-omega</h3><h4>Piotr</h4>
-
 
+
<p><ol><li>Samples were resuspended in PBS, sonicated and centrifuged.</li>  
<p><ol><li>Samples were resuspended in PBS, sonicated and centrifuged.</li>  
<li>Lysis buffer were added separately to pellet and supernatant, then samples were boiled for 10 minutes. </li>
<li>Lysis buffer were added separately to pellet and supernatant, then samples were boiled for 10 minutes. </li>
-
<li>Lysates loaded on 12% poliacrylamide gel (amount relating to 100 ul of OD=1,0 culture)</li>
+
<li>Lysates loaded on 12% poliacrylamide gel (amount relating to 100 ul of OD=1,0 culture).</li>
-
<li>Gel stained with Coomassie Blue. Optimal induction conditions chosen</li>
+
<li>Gel stained with Coomassie Blue. Optimal induction conditions chosen.</li>
-
<li>A-alpha, Z-alpha and Z-omega inoculated in the overnight culture (Rosetta strain)</li></ol></p>
+
<li>A-alpha, Z-alpha and Z-omega inoculated in the overnight culture (Rosetta strain).</li></ol></p>
<br><br>
<br><br>

Revision as of 22:41, 12 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


Purification of proteins: A-alpha, Z-alpha and Z-omega

Piotr

  1. Samples were resuspended in PBS, sonicated and centrifuged.
  2. Lysis buffer were added separately to pellet and supernatant, then samples were boiled for 10 minutes.
  3. Lysates loaded on 12% poliacrylamide gel (amount relating to 100 ul of OD=1,0 culture).
  4. Gel stained with Coomassie Blue. Optimal induction conditions chosen.
  5. A-alpha, Z-alpha and Z-omega inoculated in the overnight culture (Rosetta strain).

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/3_September_2008"