Team:Warsaw/Calendar-Main/5 September 2008

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<h3>Purification of proteins: A-alpha, Z-alpha and Z-omega<br>Piotr</h3>
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<h3>Purification of proteins: A-alpha, Z-alpha and Z-omega</h3><h4>Piotr</h4>
<p><ol><li>Bacteria centrifuged and sonicated (according to <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#Z_a_Z_o>protocol</a>).</li>
<p><ol><li>Bacteria centrifuged and sonicated (according to <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#Z_a_Z_o>protocol</a>).</li>
<li>Sonicate centrifuged. </li>
<li>Sonicate centrifuged. </li>
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<li>In case of Z-alpha and Z-omega: supernatant discarded, pellet resuspended in ddH20, centrifuged and again resuspended in ddH20</li>
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<li>In case of Z-alpha and Z-omega: supernatant discarded, pellet resuspended in ddH20, centrifuged and again resuspended in ddH20.</li>
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<li>In case of A-alpha: pellet discarded, supernatant kept for further purification steps</li></ol></p>
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<li>In case of A-alpha: pellet discarded, supernatant kept for further purification steps.</li></ol></p>
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Revision as of 22:52, 12 October 2008

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Purification of proteins: A-alpha, Z-alpha and Z-omega

Piotr

  1. Bacteria centrifuged and sonicated (according to protocol).
  2. Sonicate centrifuged.
  3. In case of Z-alpha and Z-omega: supernatant discarded, pellet resuspended in ddH20, centrifuged and again resuspended in ddH20.
  4. In case of A-alpha: pellet discarded, supernatant kept for further purification steps.

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