Team:Warsaw/Calendar-Main/23 June 2008

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<li> Inoculation other separate transformant colonies from plates with ligations: pACYC177+OmpA_alpha pACYC177+OmpA_omega (liquid LB + kanamycin).</li>
<li> Inoculation other separate transformant colonies from plates with ligations: pACYC177+OmpA_alpha pACYC177+OmpA_omega (liquid LB + kanamycin).</li>
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<li> PCR on isolated plasmids with [???]</li></ol>
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<h3>PCR results<br>
<h3>PCR results<br>

Revision as of 20:23, 9 October 2008

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Preparation of constructs with OmpA protein fusions
Michał K.

  1. Inoculation other separate transformant colonies from plates with ligations: pACYC177+OmpA_alpha pACYC177+OmpA_omega (liquid LB + kanamycin).

PCR results
Michał L., Ewa, Marcin

We have successfully amplified Omega-linker, but were unable to obtain the other two. PCRs were repeated. New annealing temp.: 48°C. Mg2+ concentration elevated to 12 μM.

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