Team:Warsaw/Calendar-Main/23 June 2008

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<h3>Preparation of alpa-A and omega-A fusions<br>
<h3>Preparation of alpa-A and omega-A fusions<br>
Michał L., Ewa, Marcin</h3>
Michał L., Ewa, Marcin</h3>
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<p>We have successfully amplified Omega-linker, but were unable to obtain the other two. PCRs were repeated. New annealing temp.: 48&deg;C. Mg<sup>2+</sup> concentration elevated to 12 μM.</p>
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<p>We have successfully amplified Omega-linker, but were unable to obtain the other two. PCRs were repeated. New annealing temp.: 48&deg;C. Mg<sup>2+</sup> concentration elevated to 4 mM.</p>
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Preparation of constructs with OmpA protein fusions
Michał K.

  1. Inoculation other separate transformant colonies from plates with ligations: pACYC177+OmpA_alpha pACYC177+OmpA_omega (liquid LB + kanamycin).

Preparation of alpa-A and omega-A fusions
Michał L., Ewa, Marcin

We have successfully amplified Omega-linker, but were unable to obtain the other two. PCRs were repeated. New annealing temp.: 48°C. Mg2+ concentration elevated to 4 mM.

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