Team:Warsaw/Calendar-Main/25 June 2008

From 2008.igem.org

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<li> Repetition of PCRs and gel-out isolation from </li>
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<li> Repetition of PCRs and gel-out isolations from <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/16_June_2008">16 June</a></li></ol>
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<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_purification_after_enzymatic_reaction">Clean-up</a> of digested PCR product. </li>
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<li> Gel electrophoresis of digested pMPMT5-AID and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper band (4800 bp)</li>
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<li> Overnight <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">ligation</a> of purified DNA fragments</li></ol>
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Revision as of 21:00, 9 October 2008

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Preparation of constructs with OmpA protein fusions
Michał K.

  1. Repetition of PCRs and gel-out isolations from 16 June

PCR of Alpha-link and link-A
Michał L. Marcin and Ewa

  1. We've ran gradient PCR to obtain Alpha-link and link-A.
    Annealing temp 48°C - 55°C.
    Elongation time 1 - 2 h.
  2. We have successfully amplified Alpha-link and link-A. Now its time for PCL (Polymerase Chain Ligation) to create fusions Alpha-A and Omega-A.

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