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Team:Warsaw/Calendar-Main/26 June 2008
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| - | <h3>Preparation of | + | <h3><h3>Preparation of constructs with OmpA protein fusions <br>Michał K.</h3> |
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<li> Repetition of PCRs, gel-out isolations and digests from <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/17_June_2008">17 June.</a></li></ol> | <li> Repetition of PCRs, gel-out isolations and digests from <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/17_June_2008">17 June.</a></li></ol> | ||
Revision as of 21:16, 9 October 2008
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Michał K. Michał L., Ewa, Marcin |
| Product | Templates | Primers | Product length |
|---|---|---|---|
| Alpha-A fusion | Alpha-linker + linker-A | AlphaL+SacI + AP+NotI | 1500 bp |
| Omega-A fusion | Omega-linker + linker-A | OmegaL+SacI + AP+NotI | 1440 bp |
PCL program for fusion A with alpha and omega
| Temperature | Time | |
|---|---|---|
| 94°C | 4:00 | |
| 94°C | 0:30 | 28 cycles |
| 48°C-62°C | 0:45 | |
| 72°C | 2:30 | |
| 72°C | 10:00 | |
| 4°C | infinite |
