Team:Warsaw/Calendar-Main/3 July 2008

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Colonies of each transformants from previous day (pET15b+OmpA_alpha and pET15b+OmpA_omega) were inoculated to liquid LB + ampicillin.</li></ol>
Colonies of each transformants from previous day (pET15b+OmpA_alpha and pET15b+OmpA_omega) were inoculated to liquid LB + ampicillin.</li></ol>
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<h3>Cloning alpha-A and omega-A fusions on pKS</h3>
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<h4>Michał L., Ewa, Marcin:</h4>
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<p>We have received sequencing results A-alpha fusion sequence is OK but in case of Omega-A the result is weird. It seems that there were error in synthesis of OmegaP+link10+homo2 primer which causes frameshift and cloning of this fusion has to be repeated from the beginning.</p>
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Revision as of 13:37, 10 October 2008

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Preparation of constructs with OmpA protein fusions
Paweł

  1. Colonies of each transformants from previous day (pET15b+OmpA_alpha and pET15b+OmpA_omega) were inoculated to liquid LB + ampicillin.

Cloning alpha-A and omega-A fusions on pKS

Michał L., Ewa, Marcin:

We have received sequencing results A-alpha fusion sequence is OK but in case of Omega-A the result is weird. It seems that there were error in synthesis of OmegaP+link10+homo2 primer which causes frameshift and cloning of this fusion has to be repeated from the beginning.

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