Revision as of 12:23, 11 June 2008

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The protocols we used

(estimated time: 2 hours and 15 min)

Materials needed:

@@ Line 34: / Line 34: @@
 *'''Roche'''
-*'''Pre-warmed at bath'''
+*'''Pre-warmed at 37°C bath'''
 *'''Cut and gel-extracted vector'''
 <br>
-*Add the proper amount of 10X buffer to a final concentration of 1X (e.g. 2 µl of 10X buffer in a final volume of 20 µl).
+*
-*Add 1 µl of Antarctic Phosphatase (up to 5 µg of cut vector).
-*Incubate at 37°C for 1 hour (Antarctic Phosphatase works).
-*Incubate at 65°C for 15 min (Antarctic Phosphatase inactivation).
 <br>