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Team:Warsaw/Calendar-Main/6 May 2008
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| - | <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#genomic_DNA_isolation"> Isolation of genomic DNA</a> (<i>E.coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#rosetta">Rosetta</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a>) - template DNA for PCR to obtain T7 polymerase DNA. DNA isolated from <i>E. coli</i> TOP10 strain was used as a negative control.<br> | + | <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#genomic_DNA_isolation"> Isolation of genomic DNA</a> (<i>E.coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#rosetta">Rosetta</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a>) - template DNA for PCR to obtain T7 polymerase DNA. DNA isolated from <i>E. coli</i> TOP10 strain was used as a negative control for PCR.<br> |
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Revision as of 08:43, 11 October 2008
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Preparation of pMPMT5+AID constructPiotr:
Isolation of genomic DNAMichał L.:
Isolation of genomic DNA (E.coli Rosetta and TOP10) - template DNA for PCR to obtain T7 polymerase DNA. DNA isolated from E. coli TOP10 strain was used as a negative control for PCR. 
Probes of isolated genomic DNA after mechanical destruction. 1 and 3-E.coli Rosetta DNA; 2-DNA ladder; 4-E.coli TOP10 DNA.
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