Team:Warsaw/Calendar-Main/18 June 2008

(Difference between revisions)

Revision as of 10:57, 11 October 2008


	Attribution analyzer / Interactive list of topics

Isolation of pACYC177.
Digest of purified pACYC177 with NdeI and BamHI (Tango 2x buffer).
Gel electrophoresis and isolation of proper band (3200 bp).
Clean-up of OmpA_alpha and OmpA_omega digest products.
Ligation of purified DNA (pACYC177 with OmpA_alpha and OmpA_omega DNA fragments).
Transformation of E.coli TOP10 with ligation products.
Plating transformants on LB+tetracycline.

Counting of blue colonies. Same results as on 12/06/2008.

@@ Line 5: / Line 5: @@
 <h4>Michał K.</h4>
 <ol><li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of pACYC177.</li>
-<li><a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">Digest</a> of purified pACYC177 with NdeI and BamHI. </li>
+<li><a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">Digest</a> of purified pACYC177 with NdeI and BamHI (Tango 2x buffer). </li>
 <li> Gel electrophoresis and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_purification_after_enzymatic_reaction">isolation</a> of proper band (3200 bp).</li>
 <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">Clean-up</a> of OmpA_alpha and OmpA_omega digest products. </li>
@@ Line 12: / Line 12: @@
 <li>Plating transformants on LB+tetracycline.</li></ol>
 <h3> Blue/white and rifampicin test </h3>
-<h4>Michał L., Ewa, Marcin:</h4>
+<h4>Michał L., Ewa, Marcin</h4>
 <p>Counting of blue colonies. Same results as on <a href=https://2008.igem.org/Team:Warsaw/Calendar-Main/12_June_2008>12/06/2008</a>. </p>