Team:Warsaw/Calendar-Main/6 July 2008

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<h3>Cloning omega-A fusion on pKS (second attempt)</h3>
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<h4>Michał L., Ewa, Marcin:</h4>
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<li>Gel electrophoresis of PCR products</li>
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<li>Gel-out of proper bands</li>
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<li>PCL reaction to create omega-A fusion:<br>
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<tr><th>Product</th><th>Templates</th><th>Primers</th><th>Product length</th></tr>
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<tr><th>Omega-A fusion</th><td>Omega-linker + linker-A</td><td>OmegaL+SacI + AP+NotI </td><td>1440 bp</td></tr>
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<tr><th colspan="3"><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcl">PCL</a> program for omega-A fusion</th></tr>
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<tr><th>Temperature</th><th>Time</th></tr>
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<tr><td>94&deg;C</td><td>4:00</td></tr>
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<tr><td>94&deg;C</td><td>0:30</td><td rowspan="3">28 cycles</td></tr>
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<tr><td>48&deg;C-58&deg;C</td><td>0:45</td></tr>
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<tr><td>68&deg;C</td><td>2:00</td></tr>
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<tr><td>68&deg;C</td><td>10:00</td></tr>
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<tr><td>4&deg;C</td><td>infinite</td></tr>
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<br/></li>
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<li>Gel electrophoresis of PCL products</li>
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<li>We have obtained no PCL product (weird?)</li>
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Latest revision as of 14:12, 11 October 2008

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