Team:Warsaw/Calendar-Main/3 June 2008

(Difference between revisions)

Revision as of 23:20, 11 October 2008


	Attribution analyzer / Interactive list of topics

DNA (PCR products from previous day) gel electrophoresis and isolation of DNA from proper band (20 cycles - 3300 bp). Fig. 1.

Digest of pMPMT5-AID+T7 (transcription fusion) with EcoRHI and HindIII (2x Tango buffer).
Use of T4 polymerase for blunting (3 hr of incubation).
DNA gel electrophoresis.
Isolation of DNA from proper band (7000 bp).
Ligation of isolated DNA (pMPM-T5 + T7 RNA-polymerase).
Transformation of E.coli TOP10 with ligation product.
Plating transformants on LB + tetracycline.

Fig. 1. PCR results of translational fusion for the pMPMT5-AID+AIDT7 construct. Lane 1 - 25 cycles, lane 2 - 20 cycles.

@@ Line 6: / Line 6: @@
 <h4>Michał K.</h4>
 <p>
-DNA (PCR products from previous day) gel electrophoresis and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">isolation</a> of DNA from proper band (20 cycles - 3300 bp). Fig. 1. </p>
+DNA (PCR products from previous day) gel electrophoresis and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">isolation</a> of DNA from proper band (20 cycles - 3300 bp). <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/3_June_2008#fig1">Fig. 1</a>. </p>
@@ Line 24: / Line 24: @@
-<img src="https://static.igem.org/mediawiki/2008/0/08/Tranlacyjna_pcr_29_05_2008.jpg">
+<a name="fig1"><img src="https://static.igem.org/mediawiki/2008/0/08/Tranlacyjna_pcr_29_05_2008.jpg"></a>
 <var><b>Fig. 1.</b> PCR results of translational fusion for the pMPMT5-AID+AIDT7 construct.<br> Lane 1 - 25 cycles, lane 2 - 20 cycles.</var>