Team:Warsaw/Calendar-Main/18 July 2008

From 2008.igem.org

(Difference between revisions)
Line 20: Line 20:
<p><ol><li> 4 colonies from transformation pACYC177+OmpA_Z_alpha were inoculated to liquid LB broth with kanamycin </li></ol></p>
<p><ol><li> 4 colonies from transformation pACYC177+OmpA_Z_alpha were inoculated to liquid LB broth with kanamycin </li></ol></p>
<br>
<br>
-
 
-
 
-
<h3>Cloning of protein Z DNA to OmpA constructs<br>Michał K.</h3>
 
-
<p><ol>
 
-
<li> Isolation of plasmids from cultures inocluated on previous day (pACYC177+OmpA_Z_alpha). </li>
 
-
<li> Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of isolated plasmids with BamHI and SacI (we found 4 good clones).</li></ol></p>
 
-
 

Revision as of 17:40, 11 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


Cloning of protein A DNA to OmpA constructs

  1. Colony PCR on colonies from plates with transformations pACYC177+OmpA_A_omega and pACYC177+OmpA_A_alpha. Primers used: AL+SacI and AP+NotI
  2. Confirmed transformant colonies (found only on pACYC177+OmpA_A_alpha plate) inoculated to liquid LB with kanamycin.

Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA
Paweł

  1. Ligation transformed into TOP10 strain and plated on LB+kanamycin

Cloning of protein Z DNA to OmpA constructs

Michał K.

  1. 4 colonies from transformation pACYC177+OmpA_Z_alpha were inoculated to liquid LB broth with kanamycin


Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/18_July_2008"