Team:Warsaw/Calendar-Main/17 July 2008

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Revision as of 17:26, 11 October 2008


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Digest and dephosphorylation (with CIAP) of pACYC177+OmpA_alpha with SacI and NotI (BamHI buffer).
Gel eloctrophoresis and gel-out (4300 bp band).
Ligation of isolated DNA fragment and Z DNA fragment isolated on 10 July.
Transformation of E. coli TOP10 strain with ligation.
Transformants plating on LB + kanamycin.

@@ Line 7: / Line 7: @@
 <h3>Cloning of protein Z DNA to OmpA constructs<br>Michał K.</h3>
 <p><ol>
-<li> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">Digest</a> and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing">dephosphorylation (with CIAP)</a> of pACYC177+OmpA_alpha with SacI and NotI. </li>
+<li> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">Digest</a> and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing">dephosphorylation (with CIAP)</a> of pACYC177+OmpA_alpha with SacI and NotI (BamHI buffer). </li>
 <li> Gel eloctrophoresis and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> (4300 bp band). </li>
 <li>