Team:Warsaw/Calendar-Main/18 July 2008

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<h3>Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA<br>
<h3>Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA<br>
Paweł</h3>
Paweł</h3>
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<p><ol><li>Ligation <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">transformed</a> into <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> strain and plated on LB+kanamycin</li>
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<p><ol><li>Ligation <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">transformed</a> into <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> strain and plated on LB + ampicillin.</li>
</ol></p>
</ol></p>
<h3>Cloning of protein Z DNA to OmpA constructs</h3>
<h3>Cloning of protein Z DNA to OmpA constructs</h3>
<h4>Michał K.</h4>
<h4>Michał K.</h4>
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<p><ol><li> 4 colonies from transformation pACYC177+OmpA_Z_alpha were inoculated to liquid LB broth with kanamycin </li></ol></p>
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<p>Colonies from transformation pACYC177+OmpA_Z_alpha were inoculated to liquid LB broth with kanamycin. </p>
<br>
<br>

Revision as of 17:47, 11 October 2008

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Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA
Paweł

  1. Ligation transformed into TOP10 strain and plated on LB + ampicillin.

Cloning of protein Z DNA to OmpA constructs

Michał K.

Colonies from transformation pACYC177+OmpA_Z_alpha were inoculated to liquid LB broth with kanamycin.


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