Cloning of protein A DNA to OmpA constructs

1. Isolation of plasmids from cultures inocluated on previous day (pACYC177+OmpA_A_omega).
2. Control digest of isolated plasmids with BamHI and SacI.

Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA
Paweł

1. Results of ligation: 6 colonies grown.
2. Each colony cultured overnight in LB + ampicillin

Cloning omega-A fusion on pKS (second attempt)

Michał L., Ewa, Marcin:

1. Isolation of plasmids from transformants
2. Digest of plasmids with SacI and NotI
3. Gel electrophoresis of products
4. 2 selected clones were send to DNA sequencing lab

Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA
Paweł

1. Isolation of plasmids from cultures inocluated on previous day
2. Control digest of isolated plasmids with NdeI and NotI. Zero positive results obtained - just empty vectors
3. Another overnight ligation of pET15b-OmpA-omega (NdeI/NotI cutted) with protein Z DNA

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