Team:UC Berkeley/ProjectOverview
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=== The Experiments === | === The Experiments === | ||
- | + | ==== Testing Individual Composite Parts ==== | |
+ | # Prepro Parts | ||
+ | We made parts of the format {promoter}{rbs}{prepro}{phoA}{term}. The promoter we used was {pBad}, and we tried 3 different ribosome binding sites with each prepro. We plated the bacteria we transformed on two plates, both with p-nitrophenyl phosphate, but one had arabinose and one did not. We screened successful rbs/prepro combinations by looking for combinations which caused blue colonies on the plate with arabinose and white colonies on the plate without arabinose. | ||
Revision as of 21:45, 17 June 2008
Contents |
You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing. | |
Tell us more about your project. Give us background. Use this is the abstract of your project. Be descriptive but concise (1-2 paragraphs) | |
Team Example 2 |
Home | The Team | The Project | Parts Submitted to the Registry | Modeling | Notebook |
---|
(Or you can choose different headings. But you must have a team page, a project page, and a notebook page.)
Overall project
Our project is centered around a system that will allow sound-induced lysis o E. coli.
Project Details
Part 2
The Experiments
Testing Individual Composite Parts
- Prepro Parts
We made parts of the format {promoter}{rbs}{prepro}{phoA}{term}. The promoter we used was {pBad}, and we tried 3 different ribosome binding sites with each prepro. We plated the bacteria we transformed on two plates, both with p-nitrophenyl phosphate, but one had arabinose and one did not. We screened successful rbs/prepro combinations by looking for combinations which caused blue colonies on the plate with arabinose and white colonies on the plate without arabinose.