Team:Warsaw/Calendar-Main/5 August 2008

From 2008.igem.org

(Difference between revisions)
Line 7: Line 7:
<h3> Cloning of truncated fragment of protein A</h3>  
<h3> Cloning of truncated fragment of protein A</h3>  
<h4>Piotr</h4>
<h4>Piotr</h4>
-
<p>Inoculation of some pACYC177+OmpA_alpha + deltaA and pACYC177+OmpA_omega + deltaA colonies of tranformnts.</p>
+
<ol><li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of plasmids from cultures inoculated on previous day.</li>
 +
<li> Control diegest with SacI and BamHI (BamHI buffer).</li>
 +
<li> Gel electrophoresis.</li></ol>
 +
 

Revision as of 12:53, 12 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 



Cloning of truncated fragment of protein A

Piotr

  1. Isolation of plasmids from cultures inoculated on previous day.
  2. Control diegest with SacI and BamHI (BamHI buffer).
  3. Gel electrophoresis.

Checking for presence of A on the cell membrane
Piotr

  1. Pouring bacteria by drops onto nitrocellulose
  2. Drying
  3. Blocking
  4. Anti-A antibody binding
  5. Washing
  6. Anti-rabbit antibody binding
  7. Developing with BCIP and NBT

[photo of the gel is to be placed here]


Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/5_August_2008"