Team:Warsaw/Calendar-Main/5 August 2008
From 2008.igem.org
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<h3>Preparing pACYC177+OmpA_omega_deltaA construct<h4>Michał K.</h4> | <h3>Preparing pACYC177+OmpA_omega_deltaA construct<h4>Michał K.</h4> | ||
- | <ol> | + | <ol><li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrotransform">Transformation</a> of E. coli <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> strain with ligation. </li> |
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- | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrotransform">Transformation</a> of E. coli <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> strain with ligation. </li> | + | |
<li>Transformants plating on LB + kanamycin. </li></ol> | <li>Transformants plating on LB + kanamycin. </li></ol> | ||
Revision as of 13:14, 12 October 2008
Cloning of truncated fragment of protein APiotr
Checking if OmpA_omega_A_alpha gives ampicillin resistancePiotrInoculation OmpA_omega_A_alpha from various IPTG concentrations (in mmol/mL) (0, 0.1, 0.25, 0.5, 0.75, 1) into same IPTG concentrations, but with various ampicillin concentrations (in mmol/mL)(25, 50, 75, 100) in ratio: 1:50. Measurement of bacterial culture growth (OD) in the evening:
Inoculation of OmpA_omega_A_alpha into various IPTG concentrations: 0, 0.1, 0.25, 0.5, 0.75 mmol/mL (replication is necessary) Checking OmpA_omega_A_alpha and OmpA_A_alpha expressionPiotr
Michał L., Ewa, Marcin
Preparing pACYC177+OmpA_omega_deltaA construct |