Team:Warsaw/Calendar-Main/5 August 2008
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<h3>Preparing pACYC177+OmpA_omega_deltaA construct<h4>Michał K.</h4> | <h3>Preparing pACYC177+OmpA_omega_deltaA construct<h4>Michał K.</h4> |
Revision as of 13:28, 12 October 2008
Cloning of truncated fragment of protein APiotr
Checking if OmpA_omega_A_alpha gives ampicillin resistancePiotrInoculation OmpA_omega_A_alpha from various IPTG concentrations (in mmol/mL) (0, 0.1, 0.25, 0.5, 0.75, 1) into same IPTG concentrations, but with various ampicillin concentrations (in mmol/mL)(25, 50, 75, 100) in ratio: 1:50. Measurement of bacterial culture growth (OD) in the evening:
Inoculation of OmpA_omega_A_alpha into various IPTG concentrations: 0, 0.1, 0.25, 0.5, 0.75 mmol/mL (replication is necessary) Checking OmpA_omega_A_alpha and OmpA_A_alpha expressionPiotr
Preparing pACYC177+OmpA_omega_deltaA construct |