Team:Warsaw/Calendar-Main/21 May 2008

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Revision as of 11:39, 19 October 2008


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Inoculation of separate transformant colonies for transcriptional fusion (liquid LB + tetracycline).
Inoculation of separate transformant colonies for translational fusion (liquid LB + tetracycline).

Strain	Inducer / repressor	Number of colonies on rifampicin
Top10	none	12
Top10	0.5 mM IPTG	17
Top10 pMPM-AID	none	13
Top10 pMPM-AID	0.1% Glucose	18
Top10 pMPM-AID	0.1% Arabinose	54
Top10 pTRC-AID	none	14
Top10 pTRC-AID	1% Glucose	17
Top10 pTRC-AID	0.5 mM IPTG	53

Conclusion: AID seems to work!

@@ Line 17: / Line 17: @@
 <tr><th>Strain</th><th>Inducer / repressor</th><th>Number of colonies on rifampicin</th></tr>
 <tr><th>Top10</th><td>none</td><td>12</td></tr>
-<tr><th>Top10</th><td>1mM IPTG</td><td>17</td></tr>
+<tr><th>Top10</th><td>0.5 mM IPTG</td><td>17</td></tr>
 <tr><th>Top10 pMPM-AID</th><td>none</td><td>13</td></tr>
 <tr><th>Top10 pMPM-AID</th><td>0.1% Glucose</td><td>18</td></tr>
@@ Line 23: / Line 23: @@
 <tr><th>Top10 pTRC-AID</th><td>none</td><td>14</td></tr>
 <tr><th>Top10 pTRC-AID</th><td>1% Glucose</td><td>17</td></tr>
-<tr><th>Top10 pTRC-AID</th><td>0.1% Arabinose</td><td>53</td></tr>
+<tr><th>Top10 pTRC-AID</th><td>0.5 mM IPTG</td><td>53</td></tr>
 </table>
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