Preparation of pMPMT5-AID+T7 and pMPMT5+AID-T7

Michał K.

1. Isolation of hypothetical pMPM-T5 with transcriptional fusion.
2. Isolation of hypothetical pMPM-T5 with translational fusion.
3. Control digest of pMPM-T5 with transcriptional fusion with EcoRI (EcoRI buffer).
4. Control digest of pMPM-T5 with translational fusion with BamHI (BamHI buffer).
5. Gel electrophoresis (Fig. 1 and Fig. 2).
6. Choice of proper clones and preparation for sequencing.

Rifampicin test

Michał L., Ewa, Marcin

1. Inoculation of liquid LB with E.coli TOP10 carrying pMPM-T5+AID
2. Inoculation of liquid LB with E.coli TOP10 carrying pTrc99A+AID