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Team:Warsaw/Calendar-Main/20 August 2008
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<p> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrotransform">Transformation</a> of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> strain with ligation products from previous day. </p> | <p> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrotransform">Transformation</a> of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> strain with ligation products from previous day. </p> | ||
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| + | <h3>Cloning of alpha to pACYC177+OmpA_A_omega and pACYC177+OmpA_Z_omega</h3><h4>Michał K.</h4> | ||
| + | <p> Some colonies from both plates(pACYC177+OmpA_alpha_A_omega and pACYC177+OmpA_alpha_Z_omega) was inoculated to liquid LB with kanamycin.</p> | ||
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Cloning of protein A DNA to GeneArt plasmidAntoniTransformation of E. coli TOP10 strain with ligation products from previous day. Cloning of alpha to pACYC177+OmpA_A_omega and pACYC177+OmpA_Z_omegaMichał K.Some colonies from both plates(pACYC177+OmpA_alpha_A_omega and pACYC177+OmpA_alpha_Z_omega) was inoculated to liquid LB with kanamycin.
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