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Team:Warsaw/Calendar-Main/27 August 2008
From 2008.igem.org
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<h3>Cloning of protein Z DNA to pET15b+Omp-alfa plasmid</h3><h4>Antoni</h4> | <h3>Cloning of protein Z DNA to pET15b+Omp-alfa plasmid</h3><h4>Antoni</h4> | ||
<p> Transformant colonies inoculated to liquid LB with ampicillin.</p> | <p> Transformant colonies inoculated to liquid LB with ampicillin.</p> | ||
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| + | <h3>Cloning of protein A DNA to pET15b+Omp-alfa plasmid</h3><h4>Michał K.</h4> | ||
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| + | <ol><li> Digestion of pGeneart+A and pET15b+Omp-alfa with NdeI and NotI (Orange buffer), pET15b was also dephosphorylated.</li> | ||
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Cloning of protein Z DNA to pET15b+Omp-alfa plasmidAntoniTransformant colonies inoculated to liquid LB with ampicillin. Cloning of protein A DNA to pET15b+Omp-alfa plasmidMichał K.
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