Team:Warsaw/Calendar-Main/25 August 2008

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<h3>Cloning of alpha to pACYC177+OmpA_A_omega and pACYC177+OmpA_Z_omega</h3><h4>Michał K.</h4>
<h3>Cloning of alpha to pACYC177+OmpA_A_omega and pACYC177+OmpA_Z_omega</h3><h4>Michał K.</h4>
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<p> Colonies from both plates(pACYC177+OmpA_alpha_A_omega and pACYC177+OmpA_alpha_Z_omega) was inoculated to liquid LB with kanamycin (only two colonies grown on each plate).</p>
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<li>  <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of digested vectors from <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/14_August_2008">14 August</a> and alpha DNA fragment from <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/19_August_2008">19 August</a> (more vectors added).</li>
 
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<li>  <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrotransform">Electroporation</a> of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a>  with ligations products.</li>
 
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<li>  Transformants plating on LB + kanamycine.</li></ol>
 
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Revision as of 23:15, 13 October 2008

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Cloning of protein A DNA to pET15b+Omp-alpha plasmid

Antoni

  1. Transformation of E. coli TOP10 strain with friday's ligation products.
  2. Transformants plating on LB + ampicillin.

Cloning of alpha to pACYC177+OmpA_A_omega and pACYC177+OmpA_Z_omega

Michał K.

Colonies from both plates(pACYC177+OmpA_alpha_A_omega and pACYC177+OmpA_alpha_Z_omega) was inoculated to liquid LB with kanamycin (only two colonies grown on each plate).

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