Team:Warsaw/Calendar-Main/9 September 2008

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<h3>Purification of proteins:  A-alpha, Z-alpha and Z-omega</h3><h4>Piotr</h4>  
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<h3>Purification of proteins:  A-alpha</h3><h4>Piotr</h4>  
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<p>Measurement of concentration of all proteins (<a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#bca">BCA method</a>). </p>
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<p>Measurement of concentration of protein(<a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#bca">BCA method</a>). </p>
<h3>Tests for ampicillin resistance given by protein added to medium</h3><h4>Piotr</h4>
<h3>Tests for ampicillin resistance given by protein added to medium</h3><h4>Piotr</h4>

Revision as of 21:31, 19 October 2008

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Purification of proteins: A-alpha

Piotr

Measurement of concentration of protein(BCA method).

Tests for ampicillin resistance given by protein added to medium

Piotr

Inoculation of all variants of OmpA proteins to LB with inductor (0.25 mM IPTG) and kanamycin.