Team:Warsaw/Calendar-Main/12 September 2008
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<h3>Tests for ampicillin resistance given by protein added to medium</h3><h4>Piotr</h4> | <h3>Tests for ampicillin resistance given by protein added to medium</h3><h4>Piotr</h4> | ||
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<li>Checking resistance for ampicillin concentrations: 50, 75 i 100 μg/ml (50 - fast growth, 75 - slower growth, 100 - bacteria grow slightly, then aggregate).</li></ol> | <li>Checking resistance for ampicillin concentrations: 50, 75 i 100 μg/ml (50 - fast growth, 75 - slower growth, 100 - bacteria grow slightly, then aggregate).</li></ol> | ||
+ | <h3>Repetition of test for ampicillin resistance given by protein added to medium</h3><h4>Michał L., Ewa, Marcin</h4> | ||
+ | <ol><li>Inoculation of 50 μl of previous-day culture into 3 ml liquid LB with kanamycin, 50 μg/ml ampicillin, IPTG and purified "prey" protein.</li><li>Overnight incubation.</li></ol> | ||
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Revision as of 13:55, 18 October 2008