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Team:Hawaii/Notebook/2008-06-11
From 2008.igem.org
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===Make more LB+Amp plates=== | ===Make more LB+Amp plates=== | ||
:<strong>Krystle</strong> | :<strong>Krystle</strong> | ||
| + | |||
| + | ===Thaw Test=== | ||
| + | :'''Norman''' | ||
| + | :* take final picture of thawing post thawing (~1wk). Writeup [[Team:Hawaii/PCC6803_Cryostock_Thaw_Test|discussion about the experiment]]. We have OK cryopreserved cells although it does not grow back very fast. For more thorough test we need to measure OD, as well as optimal conditions... (CO2 incubator!!!) Right now at least we know we have viable frozen cells. | ||
== Drylab Work == | == Drylab Work == | ||
===Standardized Notebook entries on the Wiki=== | ===Standardized Notebook entries on the Wiki=== | ||
| - | :<strong> Krystle</strong> | + | :<strong>Krystle</strong> |
| + | |||
| + | ===Fix Internal Wiki=== | ||
| + | :'''Norman''' | ||
| + | :fixed permission problems with wiki on internal prestinglab server. If you have trouble accessing it let me know. | ||
== Discussion == | == Discussion == | ||
Revision as of 07:05, 12 June 2008
Things we did today
Wetlab work
Tri-Parental Conjugation Preparation
- Krystle
- Innoculated E.coli into 40mL SOB in Erlenmeyer flasks, placed in 37oC incubator @ 250rpm
- Made 165mL BG-11 for BG-11 washes
- See experiment for details.
Make more LB+Amp plates
- Krystle
Thaw Test
- Norman
- take final picture of thawing post thawing (~1wk). Writeup discussion about the experiment. We have OK cryopreserved cells although it does not grow back very fast. For more thorough test we need to measure OD, as well as optimal conditions... (CO2 incubator!!!) Right now at least we know we have viable frozen cells.
Drylab Work
Standardized Notebook entries on the Wiki
- Krystle
Fix Internal Wiki
- Norman
- fixed permission problems with wiki on internal prestinglab server. If you have trouble accessing it let me know.
Discussion
Quote of the Day
I'll just ask Grace later. She speaks Krystle-language, meaning we save a lot of time because she already understands that I think weird and will adjust her explanation accordingly - Krystle to Norman about designing primers
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
