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Team:Warsaw/Calendar-Main/30 June 2008
From 2008.igem.org
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| - | <h3>Change of the reporter from | + | <h3>Change of the reporter from <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC320</a> with B-galactosidase to GFP or RFP</h3> |
<h4>Piotr, Weronika</h4> | <h4>Piotr, Weronika</h4> | ||
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<li>Inoculation of liquid LB medium + Amp with white colonies</li> | <li>Inoculation of liquid LB medium + Amp with white colonies</li> | ||
| - | <li>Inoculation of 10ml LB with E.coli TOP10 carrying | + | <li>Inoculation of 10ml LB with E.coli TOP10 carrying <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC320</a></li> |
<li>Inoculation of E.coli TOP10 carrying nazwa (Gfp genetrator), nazwa (RFP generator) | <li>Inoculation of E.coli TOP10 carrying nazwa (Gfp genetrator), nazwa (RFP generator) | ||
Revision as of 16:06, 24 October 2008
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Change of the reporter from pZC320 with B-galactosidase to GFP or RFPPiotr, Weronika
Cloning alpha-A and omega-A fusions on pKSMichał L., Ewa, MarcinInoculation of liquid LB medium with 6 transformant colonies. Preparation of constructs with OmpA protein fusionsPawełInoculation of E. coli with pET15b plasmid on LB + ampicillin.
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