"
Team:Illinois/Project
From 2008.igem.org
(→Antibody Receptor Tyrosine Kinase Fusion) |
m |
||
| Line 28: | Line 28: | ||
If you were not present at the June 10th meeting, pick a group and contact the members. | If you were not present at the June 10th meeting, pick a group and contact the members. | ||
| - | === Antibody GPCR Fusion === | + | === [[Team:Illinois/Antibody_GPCR_Fusion|Antibody GPCR Fusion]] === |
| + | This team will attempt pathogen detection by fusing the appropriate antibody domain to a GPCR receptor and upon binding of the target protein (i.e. cholera toxin), linking the resulting signal cascade to transcription of a reporter, GFP or LacI. | ||
Revision as of 01:00, 13 June 2008
| Home | The Team | Notebook | Research Articles | Protocols | The Project | Pictures | Parts Submitted to the Registry |
|---|
| We are the inaugural iGEM team here at the University of Illinois. We are very excited to be participating and look forward to a summer full of fun, learning, and hopefully a successful project. |  |
Contents |
Overall project
For our project we intend to create a yeast-based biosensor capable of detecting and signalling the presense of a pathogen. We intend to use directed evolution to develop a cell surface receptor capable of binding the pathogen. This receptor will then initiate a signaling cascade which will induce a change in the yeast, a color change for example. This project is important because there is a demonstrated need for cheap and effective monitoring of water-borne pathogens in the third world where traditional detection methods are not feasible.
Subprojects
To improve our odds of achieving our project we have decided to modulate our team into 3 disparate groups, each pursuing variant methods to accomplish the above goal, that is, cheap detection of water-bourne pathogens. The three methods and teams are detailed below.
If you were not present at the June 10th meeting, pick a group and contact the members.
Antibody GPCR Fusion
This team will attempt pathogen detection by fusing the appropriate antibody domain to a GPCR receptor and upon binding of the target protein (i.e. cholera toxin), linking the resulting signal cascade to transcription of a reporter, GFP or LacI.
Antibody Receptor Tyrosine Kinase Fusion
This project will attempt to detect a pathogen by using a secreted antibody or antibody fusion protein.
Bimolecular Fluorescence Biosensor
A soluble biosensor that activates a fluorescent protein upon binding a specific biomolecule
Results
| Home | The Team | Notebook | Research Articles | Protocols | The Project | Pictures | Parts Submitted to the Registry |
|---|
