From 2008.igem.org
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| - | <h3>Repetition of test for ampicillin resistance given by protein added to medium</h3><h4>Michał L., Ewa, Marcin</h4>
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| - | <p>Results of the test:</p><br>
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| - | <table id="result">
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| - | <tr><th>OmpA variant ("hunter")</th><th>"Prey" variant</th><th>Growth with prey</th><th>Growth without prey</th></tr>
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| - | <tr><th>OmpA-alpha</th><td>His+Z+omega</td><td>no</td><td>no</td></tr>
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| - | <tr><th>OmpA-alpha</th><td>His+Z+omega</td><td>no</td><td>no</td></tr>
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| - | <tr><th>OmpA-A-alpha</th><td>His+Z+omega</td><td>yes</td><td>no</td></tr>
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| - | <tr><th>OmpA-Z-omega</th><td>His+A+alpha</td><td>yes</td><td>no</td></tr>
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| - | <tr><th>OmpA-omega-A-alpha</th><td>His+Z+omega</td><td>yes</td><td>no</td></tr>
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| - | <tr><th>Ompa-A-omega</th><td>His+Z+alpha</td><td>yes</td><td>no</td></tr>
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| - | <tr><th>Ompa-A-omega</th><td>His+Z+alpha</td><td>yes</td><td>no</td></tr>
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| - | <tr><th>OmpA-omega</th><td>His+A+alpha</td><td>no</th><td>no</td></tr>
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| - | <tr><th>Ompa-Z-alpha</th><td>His+A+omega</td><td>yes</td><td>no</td></tr>
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| - | <tr><th>Ompa-Z-alpha</th><td>His+A+omega</td><td>yes</td><td>no</td></tr>
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| - | </table>
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| - | <br>
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| - | <p>Conclusions:
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| - | <li>None of tested constructs give resistance to ampicillin without prey protein added to culture medium.</li>
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| - | <li>Interaction between hunter and prey proteins connected to beta-lactamase makes cells ampicillin resistant</li>
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| - | </ol>
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| - | </p>
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Revision as of 15:20, 24 October 2008
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