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Team:Warsaw/Calendar-Main/19 June 2008
From 2008.igem.org
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<h4>Piotr</h4> | <h4>Piotr</h4> | ||
| - | <li>The analysis of lacZ sequence from pZC<br> | + | <li>The analysis of lacZ sequence from pZC:<br> |
There is no mutation in beta-galactosidase gene. Why the colonys are white???</li> | There is no mutation in beta-galactosidase gene. Why the colonys are white???</li> | ||
<li>Colony <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a>.<br> | <li>Colony <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a>.<br> | ||
Revision as of 18:40, 19 October 2008
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Preparation of constructs with OmpA protein fusionsMichał K.Inoculation of separate transformant colonies from plates with ligations: pACYC177+OmpA_alpha pACYC177+OmpA_omega (liquid LB + kanamycin). Blue/white testPiotrThere is no mutation in beta-galactosidase gene. Why the colonys are white??? Template: DNA isolated from white colonies Primers: pZCseqL and pZCseqR
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