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Team:Warsaw/Calendar-Main/26 June 2008
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<tr><td>94°C</td><td>0:30</td><td rowspan="3">28 cycles</td></tr> | <tr><td>94°C</td><td>0:30</td><td rowspan="3">28 cycles</td></tr> | ||
<tr><td>48°C-62°C</td><td>0:45</td></tr> | <tr><td>48°C-62°C</td><td>0:45</td></tr> | ||
| - | <tr><td>68°C</td><td> | + | <tr><td>68°C</td><td>1:30</td></tr> |
<tr><td>68°C</td><td>10:00</td></tr> | <tr><td>68°C</td><td>10:00</td></tr> | ||
<tr><td>4°C</td><td>infinite</td></tr> | <tr><td>4°C</td><td>infinite</td></tr> | ||
Revision as of 18:46, 19 October 2008
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Change of the reporter from pZC with B-galactosidaze to GFP or RFPPiotr, Weronika
Preparation of constructs with OmpA protein fusionsMichał K.Repetition of PCRs, gel-out isolations and digests from 17 June. Polymerase Chain Ligation (PCL)Michał L., Ewa, Marcin
PCL program for fusion A with alpha and omega
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