"
Team:Warsaw/Calendar-Main/3 July 2008
From 2008.igem.org
(Difference between revisions)
PPrzanowski (Talk | contribs) |
PPrzanowski (Talk | contribs) |
||
| Line 20: | Line 20: | ||
<h3>Cloning alpha-A and omega-A fusions on <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pKSII%2B>pKS</a></h3> | <h3>Cloning alpha-A and omega-A fusions on <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pKSII%2B>pKS</a></h3> | ||
<h4>Michał L., Ewa, Marcin</h4> | <h4>Michał L., Ewa, Marcin</h4> | ||
| - | <p>We have received sequencing results: A is OK but in case of Alpha-A and Omega-A the result is weird. It seems that there were error in synthesis of OmegaP+link10+homo2 and AlphaP+link10+homo2 primer which causes frameshift and cloning of | + | <p>We have received sequencing results: A is OK but in case of Alpha-A and Omega-A the result is weird. It seems that there were error in synthesis of OmegaP+link10+homo2 and AlphaP+link10+homo2 primer which causes frameshift and cloning of these fusions have to be repeated from the beginning.</p> |
</html> | </html> | ||
Revision as of 18:54, 19 October 2008
 |
|
||||||||
 |
|
||||||||
Change of the reporter from pZC with B-galactosidaze to GFP or RFPPiotr, Weronika
Preparation of constructs with OmpA protein fusionsPawełColonies of each transformants from previous day (pET15b+OmpA_alpha and pET15b+OmpA_omega) were inoculated to liquid LB + ampicillin. Cloning alpha-A and omega-A fusions on pKSMichał L., Ewa, MarcinWe have received sequencing results: A is OK but in case of Alpha-A and Omega-A the result is weird. It seems that there were error in synthesis of OmegaP+link10+homo2 and AlphaP+link10+homo2 primer which causes frameshift and cloning of these fusions have to be repeated from the beginning.
|
|
