Team:Warsaw/Calendar-Main/26 August 2008

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<h3>Tests for ampicillin resistance given by protein added to medium</h3><h4>Piotr</h4>
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Purification Omp_alpha of antibiotic resistance contamination
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<p> Inoculation of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a>  careing pACTC177+OmpA_alpha to liquin LB with kanamycin and with kanamycin and ampicylin.</p>
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<h3>Cloning of protein A DNA to <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-alpha>pET15b+OmpA-alpha</a> plasmid</h3><h4>Antoni</h4>
<h3>Cloning of protein A DNA to <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-alpha>pET15b+OmpA-alpha</a> plasmid</h3><h4>Antoni</h4>
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Revision as of 19:01, 20 October 2008

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Tests for ampicillin resistance given by protein added to medium

Piotr

Purification Omp_alpha of antibiotic resistance contamination

Inoculation of E. coli TOP10 careing pACTC177+OmpA_alpha to liquin LB with kanamycin and with kanamycin and ampicylin.

Cloning of protein A DNA to pET15b+OmpA-alpha plasmid

Antoni

  1. Colony PCR on colonies from plates with transformations pGeneart+A Primers used: AP+NotI and AL+SacI.
  2. Lack of confirmed transformant colonies.

Cloning of alpha to pACYC177+OmpA_A_omega and pACYC177+OmpA_Z_omega

Michał K.

  1. Isolation of plasmids from cultures inocluated on previous day (both potential constructs).
  2. Control digest of isolated plasmids with FastBamHI and FastAcc65I (Fast Digest buffer).
  3. Gel electrophoresis - still no proper clones founded.

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