Team:Warsaw/Calendar-Main/9 September 2008

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<h3>Tests for ampicillin resistance given by protein added to medium</h3><h4>Piotr</h4>
<h3>Tests for ampicillin resistance given by protein added to medium</h3><h4>Piotr</h4>
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<p>Inoculation of TOP10 carrying OmpA_Z_alpha, OmpA_Z_omega, OmpA_alpha, OmpA_omega to LB with inductor (0.25 mM IPTG) and kanamycin.</p>
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<p>Inoculation of TOP10 carrying OmpA_Z_alpha, OmpA_Z_omega, OmpA_alpha, OmpA_omega to LB with inducer (0.25 mM IPTG) and kanamycin.</p>

Revision as of 12:40, 24 October 2008

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Purification of proteins: A-alpha

Piotr

Measurement of concentration of protein(BCA method).

Tests for ampicillin resistance given by protein added to medium

Piotr

Inoculation of TOP10 carrying OmpA_Z_alpha, OmpA_Z_omega, OmpA_alpha, OmpA_omega to LB with inducer (0.25 mM IPTG) and kanamycin.

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