User:Jec105
From 2008.igem.org
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{{Imperial/Box1|Design| | {{Imperial/Box1|Design| | ||
- | + | In order to achieve our specifications of design previously described, we require the following constructs; | |
- | + | *'''Light sensing device''' - Converting a light input into an output of PoPS, | |
+ | *'''Biomaterial production device'''- Converting a PoPS input into an output of biomaterial, | ||
+ | *'''Motility Control device''' - Converting a PoPS input into an output of motility arrest, | ||
+ | *'''Integration device''' - To allow integration and selection of our genetic constructs, | ||
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Here, AB is our antibiotic resistance cassette, ''ytvA'' is the gene controlling the light-sensing pathway, ''SB'' is the biomaterial, ''epsE'' the clutch and the 5' and 3' sections are integration sites. Light-inducible promoters are labelled with an 'L'. | Here, AB is our antibiotic resistance cassette, ''ytvA'' is the gene controlling the light-sensing pathway, ''SB'' is the biomaterial, ''epsE'' the clutch and the 5' and 3' sections are integration sites. Light-inducible promoters are labelled with an 'L'. | ||
Our basic approach was to constitutively upregulate a light-sensitive pathway in ''B. subtilis'', then use a downstream product from that pathway to selectively regulate expression of our clutch and biomaterial. This overview is shown on the right. To do this we need to produce at least one constitutive promoter to precede our light-sensing mechanism, as well as a 'light-inducible' promoter in front of our clutch and biomaterial. Additionally, we'll need to make all the other standard parts such as ribosome binding sites (RBS) and terminators. | Our basic approach was to constitutively upregulate a light-sensitive pathway in ''B. subtilis'', then use a downstream product from that pathway to selectively regulate expression of our clutch and biomaterial. This overview is shown on the right. To do this we need to produce at least one constitutive promoter to precede our light-sensing mechanism, as well as a 'light-inducible' promoter in front of our clutch and biomaterial. Additionally, we'll need to make all the other standard parts such as ribosome binding sites (RBS) and terminators. | ||
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+ | [[Image:Genetic circuit.PNG|750px|center]] | ||
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The diagram below shows our theoretical final construct once it's all put together - as you can see, it's a little more complex than the one on the right! | The diagram below shows our theoretical final construct once it's all put together - as you can see, it's a little more complex than the one on the right! |
Revision as of 22:10, 20 October 2008
Summer Summary
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