Team:Mississippi State/Timeline of Things To Do
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- | We should have the pPIC6alpha vector by Monday (June 16), along with the primers for the cDNA. Thus, we will do as follows: | + | *We should have the pPIC6alpha vector by Monday (June 16), along with the primers for the cDNA. Thus, we will do as follows: |
#As soon as we get vector, transform into E-Coli for storage | #As soon as we get vector, transform into E-Coli for storage | ||
#Make glycerol stock for storage of yeast | #Make glycerol stock for storage of yeast | ||
#PCR cDNA from P.C. to isolate LiPA using primers we have ordered. | #PCR cDNA from P.C. to isolate LiPA using primers we have ordered. |
Latest revision as of 14:54, 13 June 2008
Timeline of Things To Do
A
- Order Supplies, most importantly the yeast vector(pPIC6alpha) and the gene(one of the LiP genes).
- Contact MSU person about possibility of getting LiP here
- Research other ways of obtaining the gene
- Center for Forest Mycology in Madison, WI has the organism and possibly the gene
B
- When we get supplies we need to begin by isolating LiP
- Transform into vector and measure expression
- Construct LiP BioBrick
- Transform into vector and measure expression
- Establish control system for expression
- Develop an entire system for controlable production and secretion of LiP
C
- We should have the pPIC6alpha vector by Monday (June 16), along with the primers for the cDNA. Thus, we will do as follows:
- As soon as we get vector, transform into E-Coli for storage
- Make glycerol stock for storage of yeast
- PCR cDNA from P.C. to isolate LiPA using primers we have ordered.